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KMID : 0881720180330010058
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 1 p.58 ~ p.64
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Analytical Method for Determination of Cephalexin in Bovine Edible Tissues using Liquid Chromatography Coupled to Tandem Mass Spectrometry
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Chae Won-Seok
Lee Sung-Joong
Son Song-Ee
Kim Suk
Lee Hu-Jang
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Abstract
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An analytical method for the determination of cephalexin (CEX) in bovine tissues (muscle, liver, kidney and fat tissues) was developed and validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Tissue samples were extracted by the liquid-liquid extraction based on 5% trichloroacetic acid (TCA). The chromatographic separation was achieved on a reverse phase C18 column with gradient elution using a mobile phase of 20 mM hexafluroacetylacetone (HFAC)/50% acetonitrile (40:60). The procedure was validated according to the Ministry of Food and Drug Safety guideline determining accuracy, precision, and limit of detection. Mean recoveries of CEX from spiked edible tissues (6~1,500 μg/kg) were 83.9~106.8%, and the relative standard deviation was between 2.3 and 14.8%. Linearities were obtained with the correlation coefficient (r2) of > 0.999. Limit of detection and limit of quantification for the investigated CEX were 2~10 and 6~30 μg/kg, respectively. This method was reliable, sensitive, economical and suitable for routine monitoring of CEX residues in bovine edible tissues.
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KEYWORD
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Cephalexin , Bovine edible tissues , LC-MS/MS
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